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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Nat Struct Mol Biol. 2010 Aug 22;17(9):1102–1108. doi: 10.1038/nsmb.1887

Figure 1.

Figure 1

Pyrabactin functions both as selective ABA receptor agonist and antagonist. (a) Stimulation of PYR/PYL–ABI1 interaction by pyrabactin. PP2C interaction in the presence and absence of 100 μM pyrabactin was determined by AlphaScreen luminescence proximity assays as detailed in Methods (n=3, error bars=s.d.). (b) Pyrabactin-dependent inhibition of ABI1 phosphatase activities by PYR/PYL proteins (n=3, error bars=s.d.). (c) Pyrabactin inhibits the ABA-stimulated interaction between PYL2 and PP2Cs as determined by Alphascreen assays in the presence or absence of 10 μM (+)-ABA and the indicated concentrations of pyrabactin (n=3, error bars=s.d.). (d) Pyrabactin relieves the ABA-stimulated inhibition of PP2C phosphatase activity by PYL2. Reactions contained 10 μM (+)-ABA and the indicated concentrations of pyrabactin (n=3, error bars=s.d.). (e) Pyrabactin inhibits ABA-stimulated reporter gene activity in a PYL2-reconstituted ABA signaling pathway in Arabidopsis mesophyll protoplasts. Expression plasmids for PYL2, ABI1, SnRK2.6, and the transcription factor ABF2 were cotransfected into protoplasts together with a luciferase reporter plasmid driven from the ABA-responsive RD29B gene (n=3, error bars=s.d.).