Figure 2.

BRMS1 promoter is hypermethylated in lung cancer tissue when compared to normal adjacent tissue. (A) BRMS1 is hypermethylated in lung cancer tissues. Quantitative MSP for BRMS1 promoter in tumour tissues (T) and patient-matched adjacent lung tissues (N). The data are plotted as the fold over patient-matched adjacent non-cancerous lung tissues (N), where results from N were normalized to 1 for each patient. (B) The BRMS1 promoter presents higher methylation status in lung cancer compared with adjacent non-cancerous tissue. (Upper panel) Schematic illustration of the bisulphite sequence PCR target sequence in the BRMS1 promoter. The 18 bars represent CpG dinucleotides; the arrow represents the transcription start site. (Lower panel) Methylation status of the BRMS1 5′ CpG island in lung cancer samples and adjacent non-cancerous tissues. Each horizontal row of circles represents one patient sample. Each CpG dinucleotide bar from the schematic is depicted by a circle, the fill pattern of which indicates the methylated cytosine and the blank indicates the unmethylated cytosine in CpG dinucleotide. T, tumour; n = adjacent non-cancerous tissue.