Skip to main content
. Author manuscript; available in PMC: 2011 Sep 7.
Published in final edited form as: Biochemistry. 2010 Sep 7;49(35):7532–7541. doi: 10.1021/bi902026v

Table 2.

Difference between wild type AeSCP-2 and mutants in NBD-cholesterol binding in vitro

wt Y30A F9W/W44
F		 F105
A		 F105
W		 M90L W44E F32W F32A
Kd (µM)* 0.34
(0.16)		 0.25
(0.05)		 0.91 (0.62) 1.15
(0.09)		 1.32
(0.45)		 2.75
(1.45)		 4.20
(1.67)		 4.94
(1.59)		 6.36
(2.11)
P**vs. wt -- >0.05 >0.05 >0.05 >0.05 >0.05 >0.05 >0.05 <0.05
Fold of Kd
change		 0.73 2.68 3.38 3.88 8.09 12.35 14.53 18.71
*

Mean Kd values (standard deviation) determined using the GraphPad PRISM4.0 (single binding site and non-linear regression model).

**

P values were given for Dunnett's post test between wild type (wt) and each mutant after a repeated measures analysis of variance.

Net changes in NBD-cholesterol fluorescence intensity (RFU = Relative fluorescence unit) in each concentration were processed (0.5 µM NBD-cholesterol with increasing protein concentrations (0.01 to 10 µM)).