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. Author manuscript; available in PMC: 2011 Aug 27.
Published in final edited form as: Biochem Biophys Res Commun. 2010 Jul 30;399(3):425–428. doi: 10.1016/j.bbrc.2010.07.098

Fig. 3.

Fig. 3

EGF-induced intracellular calcium rise requires the catalytic activity of PLC-γ1. SCC4 cells cultured on glass coverslips were transfected with the full-length (FL) and X-domain truncated (ΔX) PLC-γ1-FLAG-tagged fusion constructs as indicated. Transfected cells were selected by 4-day incubation in 300 μM G418. Intracellular calcium was measured, and the response to EGF (50 ng/ml) was determined. The traces shown in this figure represent the average intracellular calcium of 45–60 individual cells during recording. The results shown are from a representative experiment repeated three times with three separate transfection experiments.