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. Author manuscript; available in PMC: 2011 Aug 27.
Published in final edited form as: Biochem Biophys Res Commun. 2010 Jul 30;399(3):425–428. doi: 10.1016/j.bbrc.2010.07.098

Fig. 4.

Fig. 4

EGF-induced cell migration requires the catalytic activity of PLC-γ1, IP3 and intracellular calcium. SCC4 cells were transfected with the full-length (FL) and X-domain truncated (ΔX) PLC-γ1-FLAG-tagged fusion constructs as indicated. Transfected cells were selected by 4-day incubation in 300 μM G418 and pretreated with 2-APB (50 μM), BAPTA-AM (10 μM) or vehicle, and then treated with EGF (50 ng/ml) for 16 h. Cell migration was determined by Transwell assay. The results are expressed as percentages of the values obtained with vector-transfected/vehicle-treated cells. Data are mean ± SD of triplicates within a single representative experiment, *p<0.01 (highly significantly different from the vehicle treated cells). Results shown are representative of three independent experiments.