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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Cancer Res. 2010 Jun 15;70(13):5438–5447. doi: 10.1158/0008-5472.CAN-09-2544

Figure 3. Reactivation of PP2A induces apoptosis and suppresses the clonogenic potential of mutant c-KIT cells.

Figure 3

A, FDC-P1 cells were treated with FTY720 or FTY720-P (2.5 μM; 6 h). PP2A activity was determined by incubating the isolated PP2Ac complex with a PP2A-specific phosphopeptide and measuring free phosphate release using a colorimetric assay. PP2A activity is normalised to untreated EV controls. B, FDC-P1 cells were treated with FTY720 (2.5 μM, 24 h) and assessed for annexin-V by flow cytometry. C, FDC-P1 cells were treated with FTY720 (2.5 μM; 36 h) and stained with PI to analyse DNA content. Plots are a representative of four independent experiments. D, E, FDC-P1 cells were grown in methylcellulose for 7days in the presence of indicated drugs. Columns, mean colony number (n=4); bars, SEM. *, p<0.05; **, p<0.01, Student’s t-test compared to untreated.