Figure 4. Reactivation of PP2A dephosphorylates the c-KIT receptor and its downstream signaling targets.
A, c-KIT was immunoprecipitated from FDC-P1 cells treated with 2.5 μM FTY720 (0, 6, 12, 24 h). Levels of tyrosine phosphorylated (pY) and total c-KIT were determined by immunoblotting (top). Quantitation of three independent experiments (bottom). B, Immunoblots detecting phosphorylated or total Akt, STAT5, ERK1/2, p38MAPK, MEK and BAD after FTY720 treatment (2.5μM; 12h).