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. 2009 Oct 5;43(3):276–285. doi: 10.1165/rcmb.2008-0438OC

Figure 1.

Figure 1.

Cell surface expression of maturation markers and costimulatory molecules. Bone marrow precursor–derived dendritic cells from Nrf2+/+ and Nrf2−/− mice were stimulated in vitro with ragweed extract (RWE) (50 μg /ml) for 48 hours, and surface expression of CD11c, CD40, CD80, CD86, and MHCII was measured using two-color flow cytometry. (A) Representative flow histograms from six independent experiments (n = 6 mice per group). Numbers within graph quadrants are percentage of positive cells. (B) Data shown are mean ± SEM of mean fluorescence intensity (MFI) from six independent experiments (n = 6 mice per group). *P < 0.05 when comparing the effect of RWE with medium control (resting). Significant baseline differences between genotype are not indicated in the figure but are contained in Table 1. (C) The purity of immature dendritic cells was further confirmed by staining the cells with anti-CD11c and anti–CD8-α antibodies. The immature dendritic cells from Nrf2+/+ and Nrf2−/−mice are CD11chigh and showed decreased CD8-α expression.