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. 2009 Oct 5;43(3):276–285. doi: 10.1165/rcmb.2008-0438OC

Figure 3.

Figure 3.

Effects of N-acetyl cysteine (NAC) on bone marrow precursor–derived DC (BM-DC) surface molecule expression. (A) To determine the inhibitory effect of NAC, immature DCs (Day 8) from Nrf2+/+ mice were preincubated with different concentrations of NAC (0, 1, 10, and 100 mM) for 1 hour and then cultured with RWE (50 μg/ml). After 48 hours, cells were harvested, and the surface expression of CD80 and MHCII were analyzed by flow cytometry after staining with corresponding antibodies. Results of one representative experiment of the three are shown. To determine the effect of NAC on RWE-induced maturation of DCs, BM-DCs from Nrf2+/+ or Nrf2−/− were pretreated with NAC (5 mM) for 1 hour and then exposed to RWE for 48 hours. The expression of (B) CD80, (C) CD86, and (D) MHCII was analyzed by flow cytometry. All data are mean ± SEM of MFI from three independent experiments (n = 5 mice per group). *P < 0.05 for the indicated comparisons.