Figure 4.

Photomicrographs of CFU immunostaining after culturing peripheral blood mononuclear cells for 7 days on fibronectin-coated glass slides. Scale bar = 100 μm. Red indicates VE-cadherin, green indicates α-smooth muscle actin, and blue indicates DAPI nuclear stain. (A–D) Sample from a representative healthy control subject. (E–H) Sample from a representative patient with ALI. (B, F, J ) Enlarged photographs of VE-cadherin staining, to show details. Spindle-shaped cells were evident only in patients with ALI. (I–L) Primary human microvascular pulmonary endothelial cells were used in these experiments as positive controls for VE-cadherin. (M) Murine mesenchymal stem cells were used as positive controls for α-smooth muscle actin.