Figure 1.

Expression of plasminogen activator inhibitor type–1 (PAI-1) by MeT5A human pleural mesothelial and MS-1 mesothelioma cells. Total proteins from conditioned media (CM) and cell lysates (CL) of MeT5A and MS-1 cells treated with PBS, TNF-α (10 ng/ml), or TGF-β (2 ng/ml) for 24 hours were subjected to Western blotting using an anti–PAI-1 antibody. The corresponding blots containing proteins from the CL were stripped and reprobed with β-actin monoclonal antibody for assessment of equal loading.