Table 2.

Kinetic constants for the binding of mAbs MS-A and MS-B to DNA as determined by surface plasmon resonance

mAb	kon, s−1⋅M−1	koff, s−1	Kd, nM
Synthetic oligonucleotide duplex
MS-A	1.1  × 105	4.0  × 10−4	3.6
MS-B	1.1  × 105	1.1  × 10−4	1.0
Amplified DNA fragment
MS-A	5.8  × 104	5.5  × 10−4	9.4
MS-B	6.6  × 104	1.9  × 10−4	2.9

Antibody binding was measured against a short oligonucleotide duplex and against a PCR-amplified DNA fragment of ≈700 bp in length.