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. 2010 Aug 23;10:449. doi: 10.1186/1471-2407-10-449

Figure 1.

Figure 1

Main constituents of cytoskeletons from the breast cell lines. Sections of the 2-DE gels (Mr 38-60, pI 4.6-5.7) from breast cell lines showing the main constituents of the cytoskeleton. (A) The identities of spots are shown for cell line EM-G3. Spots corresponding to CKs are framed. Simple CKs are in green, basal CKs are in blue and CKs atypical for mammary glands are in yellow. The data were subtracted from Selicharova et al. [23]. Protein names are abbreviated as 40S rib.p.SA = 40S ribosomal protein SA, CK = cytokeratin, HSP = heat shock protein and PDI = protein disulfide isomerase. (B) Estimated positions of CKs are shown for the cell lines HCC1937, MCF7 and MDA-MB-231. (C) Immunoblot analysis. Proteins from each cell lysate were separated by 1-D SDS-PAGE and detected with rabbit anti-actin antibody and mouse monoclonal anti-vimentin, anti-CK19 and anti-CK13 antibodies.