Table 3.
Proteins identified from GSH-binding fractions of breast cell lines
| Spot no. | Accession no. | Protein name | Theor. Mr/pI | Meas. Mr/pI | Seq. cov. (%) | Pept. seq. |
|---|---|---|---|---|---|---|
| 1 | P09211 | GST P1-1 | 23,2/5,4 | 25/5,5 | 52 | 15 |
| 2 | P09211 | GST P1-1 | 23,2/5,4 | 25/5,7 | 47 | 7 |
| 3 | P09211 | GST P1-1 | 23,2/5,4 | 25/5,3 | 51 | 10 |
| 4 | P21266 | GST M3-3 | 26,4/5,4 | 27/5,6 | 33 | 8 |
| 5 | P24534 | EF 1-β | 24,6/4,5 | 30/4,5 | 24 | 6 |
| 6 | P26641 | EF 1-γ | 50,0/6,3 | 50/nda | 18 | 7 |
| 7 | P60709 | β-actin | 41,6/5,3 | 44/5,3 | 18 | 7 |
| 8 | P16152 | Carbonyl reductase [NADPH] 1 | 30,2/8,5 | 32/8,5 | 20 | 5 |
| 9 | O43813 | LanCL 1 | 45,3/7,9 | 40/nda | 5 | 2 |
For each protein spot we show the accession number in the Swiss-Prot database, the protein name or its abbreviation, the theoretical (Theor.) and experimental (Meas.) relative molecular weight (in kDa) and isoelectric point (Mr/pI), the sequence coverage and the number of peptides sequenced. Protein names are abbreviated as GST = glutathione S-transferase, EF = elongation factor, LanCL 1 = LanC-like protein 1.
a) The proteins were identified from 1-D SDS-PAGE gels, and their positions in 2-D gels were estimated according to their Mr/pI (Figure 3).