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Synthetic pathway for the preparation of the phosphoramidite derivative of endcap 2.
Reagents and conditions: i. BuLi, −78°C; ii. BF3 · Et2O, oxetane; iii. DMTrCl, pyridine; iv. 2-cyanoethyl-N, N-diisopropyl-chlorophosphoramidite, diisopropylethylamine.
