Table 3.
Oligonucleotide | Endcap Tm values (°C) | ||||||||
---|---|---|---|---|---|---|---|---|---|
2 | 8 | 22 | 25 | 29 | 32 | 33 | 34 | TTTT | |
IV | 66 | 39 | 46 | 50 | 48 | 62 | 53 | 60 | 39 |
V | 62 | 42 | 56 | 61 | 56 | 75 | 66 | 74 | 58 |
All DNA sequences are written in 5′→3′ direction. X: endcap
Oligonucleotide | Endcap Tm values (°C) | ||||||||
---|---|---|---|---|---|---|---|---|---|
2 | 8 | 22 | 25 | 29 | 32 | 33 | 34 | TTTT | |
IV | 66 | 39 | 46 | 50 | 48 | 62 | 53 | 60 | 39 |
V | 62 | 42 | 56 | 61 | 56 | 75 | 66 | 74 | 58 |
All DNA sequences are written in 5′→3′ direction. X: endcap