Table 2.
Oligonucleotide Primer Sequences for Detecting KIT, HRAS, KRAS, NRAS, BRAF, PIK3CA, and PTEN.
| Genes | Exon | Forward Primer Sequence (5′ to 3′) | Reverse Primer Sequence (5′ to 3 ′) | Expected PCR Product Size (bp) |
|---|---|---|---|---|
| KIT | 9 | TAAAAGTATGCCACATCCCAAG | CATGGTCAATGTTGGAATGAAC | 368 |
| KIT | 11 | TCCAGAGTGCTCTAATGAC | AGGTGGAACAAAACAAAGG | 292 |
| KIT | 13 | AAGATGCTCAAGCGTAAGTTC | AAGCAGTTTATAATCTAGCATTGCC | 302 |
| KIT | 17 | GTGAACATCATTCAAGGCGT | CCTTTGCAGGACTGTCAAGCA | 336 |
| HRAS | 1 | TCTAGAGGAAGCAGGAGACAGG | CTTTTCCCATCACTGGGTCAT | 515 |
| HRAS | 2 | GAGTCCTTCACCCGTTTGATCT | CCCTGTCTCCTGCTTCCTCTA | 519 |
| KRAS | 1 | GCACAGAGAGTGAACATCATGG | CATTTTTCTTAAGCGTCGATGG | 574 |
| KRAS | 2 | ACAGAAGGCTGTGGAGTCAAAC | TCATCTTTGGAGCAGGAACAAT | 574 |
| NRAS | 1 | GGACAGGTTTTAGAAACTTCAGCA | GGGAGTAATAGGAAGGGGGATT | 590 |
| NRAS | 2 | CCAAGTCATTCCCAGTAGCAAG | TTTGAGGGACAAACCAGATAGG | 568 |
| BRAF | 11 | TGAGGACTAGTTAACCTGGAGGA | TTTTTCCATCCTAATATTGACTTCAG | 581 |
| BRAF | 15 | TTTTTCAACAGGGTACACAGAACA | GCCCCAAAAATCTTAAAAGCAG | 745 |
| PIK3CA | 1 | GCCTAATCAAGTCAAACTATGGAAA | TCTAAGTCATCCCACAAATGACA | 769 |
| PIK3CA | 9 | TGGTTCTTTCCTGTCTCTGAAAA | GCATTTAATGTGCCAACTACCAA | 521 |
| PIK3CA | 20 | GCTTTGTCTACGAAAGCCTCTCT | GCAATAACAGCCTTTGTTGTGTC | 793 |
| PTEN | 2 | TCAATTTTGGGGTTTTGTTTTG | ACTGTATCCCCCTGAAGTCCAT | 563 |
| PTEN | 3 | TCTGAAAAGCTCTGGTTTTACTTCA | ATATGGGCTAGATGCCAAGTCA | 547 |
| PTEN | 4 | TGGCATCACAAGTTTTTAAGCA | AGACCAACTGCCTCAAATAGTAGG | 600 |
| PTEN | 5 | GGGGAAAATAATACCTGGCTTC | GAAGCTCTACTGGACAGCATAGTG | 798 |
| PTEN | 6 | CGCTGTTGTGACCTTTGAATAA | ATTGCTTTTGGCTTCTTTAGCC | 589 |
| PTEN | 7 | TTGAAGGTTCAAACTGGAGAAAA | AATGTCTCACCAATGCCAGAGT | 516 |
| PTEN | 8 | CATGTGAATGAAAATGCAACAGA | TGTTACTGCTACGTAAACACTGCTTC | 581 |