Table 3.
Cytokine Production in RAW264.7 Cells Untreated, NAC Only, or DMXAA-Treated with or without Previous Incubation with NAC.
| Cytokine Concentration (pg/ml)* | ||||
| Untreated | NAC | DMXAA | DMXAA + NAC | |
| Eotaxin | 6 ± 0 | 6 ± 0 | 9 ± 1 | 6 ± 0 |
| G-CSF | 7 ± 1 | 6 ± 0 | 392 ± 79 | 40 ± 24 |
| GM-CSF | 6 ± 0 | 6 ± 0 | 8 ± 1 | 8 ± 1 |
| IFN-γ | 6 ± 0 | 6 ± 0 | 9 ± 0 | 6 ± 0 |
| IL-10 | 6 ± 0 | 6 ± 0 | 6 ± 0 | 6 ± 0 |
| IL-12(p40) | 6 ± 0 | 6 ± 0 | 6 ± 0 | 6 ± 0 |
| IL-12(p70) | 6 ± 0 | 6 ± 0 | 19 ± 1 | 10 ± 1 |
| IL-13 | 6 ± 0 | 6 ± 0 | 6 ± 0 | 6 ± 0 |
| IL-15 | 6 ± 0 | 6 ± 0 | 6 ± 0 | 6 ± 0 |
| IL-17 | 6 ± 0 | 6 ± 0 | 26 ± 3 | 14 ± 3 |
| IL-1α | 6 ± 0 | 6 ± 0 | 27 ± 8 | 7 ± 0 |
| IL-1β | 19 ± 2 | 44 ± 4 | 27 ± 5 | 51 ± 6 |
| IL-2 | 7 ± 1 | 7 ± 1 | 12 ± 0 | 8 ± 1 |
| IL-3 | 6 ± 0 | 6 ± 0 | 6 ± 0 | 6 ± 0 |
| IL-4 | 6 ± 0 | 6 ± 0 | 6 ± 0 | 6 ± 0 |
| IL-5 | 6 ± 0 | 6 ± 0 | 27 ± 3 | 11 ± 1 |
| IL-6 | 6 ± 0 | 6 ± 0 | 1002 ± 129 | 309 ± 117 |
| IL-7 | 6 ± 0 | 6 ± 0 | 6 ± 0 | 6 ± 0 |
| IL-9 | 126 ± 19 | 70 ± 16 | 146 ± 2 | 92 ± 11 |
| IP-10 | 17 ± 1 | 6 ± 0 | 7609 ± 904 | 2636 ± 402 |
| KC | 6 ± 0 | 6 ± 0 | 311 ± 23 | 20 ± 4 |
| LIF | 6 ± 0 | 6 ± 0 | 11 ± 1 | 8 ± 1 |
| LIX | 6 ± 0 | 6 ± 0 | 287 ± 43 | 121 ± 44 |
| M-CSF | 6 ± 0 | 6 ± 0 | 10 ± 1 | 8 ± 2 |
| MCP-1 | 546 ± 42 | 141 ± 11 | 3781 ± 145 | 1149 ± 152 |
| MIG | 6 ± 0 | 6 ± 0 | 11 ± 4 | 6 ± 0 |
| MIP-1α | 161 ± 7 | 113 ± 3 | 25,171 ± 0 | 8766 ± 1897 |
| MIP-1β | 75 ± 5 | 29 ± 7 | 19,834 ± 5337 | 8183 ± 418 |
| MIP-2 | 300 ± 16 | 315 ± 26 | 4078 ± 347 | 2779 ± 651 |
| RANTES | 8 ± 2 | 6 ± 0 | 698 ± 13 | 39 ± 3 |
| TNF-α | 8 ± 1 | 6 ± 0 | 3052 ± 1494 | 431 ± 98 |
| VEGF | 313 ± 19 | 6 ± 0 | 355 ± 25 | 6 ± 0 |
*
Mean ± SEM of three cultures per treatment group.
GM-CSF indicates granulocyte/macrophage colony-stimulating factor; MIG, monokine induced by IFN-γ; VEGF, vascular endothelial growth factor.