Figure 2.
Quantitation of IgG secretion by two hybridomas: 707H/164 (○) and 707H/100 (●). Each hybridoma was seeded at a concentration of 3 × 105 cells per milliliter in RPMI 1640 growth medium supplemented with 10% FBS. Samples were collected at the indicated intervals for IgG quantitation by the nephelometric assay and for cell counts. After 4 days the viable cell count of the 707H/164 hybridoma reached 8 × 105 cells per milliliter, whereas that of a 707H/100 reached 6 × 105 cells per milliliter. Thereafter the number of viable cells decreased, and by the 10th day nearly all of the cells were dead when the level of IgG in the medium was at its highest, namely 210 μg/ml of IgG for both cultures.