Table 1.

Mosquito species used in this study, reactivities of actin-specific primers by RT-PCR, and GenBank accession numbers.

Species	Subfamily	Source of mosquitoes	Primer pair1			GenBank accession no.
			Act-2F + 2R	Act-2F + 3R	Act-2F + 8R
Aedes aegypti	Culicinae	YP2	+3	+	+	GQ981439
Ae. albopictus	Culicinae	ISU4	+	+	+	GQ981440
Ae. caspius	Culicinae	ISU	+	+	+	GQ981456
Ae. scapularis	Culicinae	NM5	+	+	+	GQ981441
Ae. taeniorhynchus	Culicinae	NM	+	+	+	GQ981457
Ae. triseriatus	Culicinae	ISU	+	+	+	GQ981442
Ae. trivittatus	Culicinae	ISU	+	+	+	GQ981443
Anopheles albimanus	Anophelinae	YP	+	+	+	GQ981444
An. crucians	Anophelinae	NM	+	+	+	GQ981467
An. pseudopunctipennis	Anophelinae	NM	+	+	+	GQ981445
An. quadrimaculatus	Anophelinae	NM	+	+	+	GQ981446
An. stephensi	Anophelinae	ISU	+	+	+	GQ981447
An. vestitipennis	Anophelinae	YP	+	+	+	GQ981448
Culex coronator	Culicinae	NM	+	+	+	GQ981449
Cx. erraticus	Culicinae	NM	+	+	+	GQ981450
Cx. interrogator	Culicinae	YP	+	+	+	GQ981451
Cx. nigripalpus	Culicinae	YP	+	+	+	GQ981452
Cx. pipiens	Culicinae	ISU	+	+	+	GQ981453
Cx. quinquefasciatus	Culicinae	NM	+	+	+	GQ981468
Deinocerites cancer	Culicinae	YP	+	+	+	GQ981454
Mansonia titillans	Culicinae	YP	+	+	+	GQ981455
Psorophora albipes	Culicinae	YP	+	−	+	GQ981458
Ps. cyanescens	Culicinae	YP	+	+	+	GQ981459
Ps. ciliata	Culicinae	YP	+	+	+	GQ981460
Ps. columbiae	Culicinae	NM	+	+	+	GQ981461
Ps. confinnis	Culicinae	YP	−6	+	+	GQ981462
Ps. ferox	Culicinae	YP	+	+	+	GQ981463
Ps. howardii	Culicinae	YP	+	+	+	GQ981464
Toxorhynchites amboinensis	Toxorhynchitinae	ISU	+	+	+	GQ981465
Wyeomyia spp.7	Culicinae	YP	−	+	+	GQ981466

¹Sizes of RT-PCR products generated by primer pairs Act-2F + 2R, Act-2F + 3R and Act-2F + 8R are 728, 794, and 683 nt, respectively.

²Yucatan Peninsula of Mexico.

³Positive.

⁴Insectaries at Iowa State University.

⁵Northern Mexico.

⁶Negative.

⁷Mosquitoes were identified to the genus but not species level.