Fig. 2.

Binding efficiency of ligands to D2DR and D1DR in MsrA−/− and WT membranes. Specific ligand binding was calculated from subtracting non-specific ligand binding from total ligand binding as described in ‘Materials and Methods’. (a) Representative [³H]raclopride and [³H]quinpirole specific ligand binding curves of D2DR. (b) B_max of [³H]quinpirole binding presented as percent of WT B_max value (represents 100% binding) in the absence (Control) and presence of recombinant yeast MsrA (rMsrA Added). (c) Binding of [³H]SCH23390 at a saturating concentration (B_max value WT, under these conditions, represents 100% binding). (d) Relative binding of [³H]SCH23390 at a concentration of 2 nM (as in panel c) in the presence of 2 nM and 16 nM SKF82958 concentrations. For all graphs, n = 3. Significance is denoted by * or ^† indicating p < 0.05 using t-test for difference by mouse type or by condition, respectively.