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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: J Neurochem. 2010 Mar 31;114(1):51–61. doi: 10.1111/j.1471-4159.2010.06721.x

Fig. 6.

Fig. 6

Oxidized methionine residues in D2DRL-IC3 recombinant protein. The short and long form D2DR differ by a 29-amino-acid region in the third cytoplasmic loop, which begins after amino acid 241 in the rat sequence (underlined). The methionines (m) are in bold. The corresponding recombinant fusion protein GST-D2DRL-IC3 was isolated and resulting peptides were subjected to tandem mass spectrometry after trypsin digestion. The sequence of the major oxidized peptide identified is shown and also indicated in the sequence by parenthesis. Representative relative signal levels for the daughter ions of the major peptide detected after collision-induced fragmentation are presented as relative intensity of each ion. The difference in percent methionine (Y10) oxidation between the presence and absence of rMsrA was found to be significant (three experiments, p < 0.05 by t-test).