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. Author manuscript; available in PMC: 2010 Sep 6.
Published in final edited form as: Biochemistry. 2008 Sep 5;47(39):10420–10427. doi: 10.1021/bi800904m

Table 2.

Steady-state kinetic parameters from initial velocity studies

Varied Substrate Fixed Substrate kcat
(min−1)		 Ki(pep)
(µM)		 K(pep)
(µM)		 Ki(SAM)
(µM)		 K(SAM)
SAM AcH4-21a 0.54 ± 0.01 20.2±7.3 8.67 ± 1.80 49.1 ± 11.4 21.1 ± 4.0
SAM AcH4-21 R3MMAb 1.42 ± 0.02 4.01±3.08 2.92 ± 0.96 20.2 ± 13.4 14.7 ± 2.9
AcH4-21 SAMc 0.56 ± 0.02 42.9 ± 18.5 32.5 ± 24.1 11.0 ± 9.84 8.35 ± 2.11
AcH4-21 R3MMA SAMd 0.96 ± 0.01 36.1 ± 14.4 4.26 ± 3.71 24.83± 24.44 2.93 ± 0.66
a

2.5, 5, 25 µM

b

2.5, 5, 10, 25, 125 µM

c

1, 2.5, 7.5 µM

d

2.5, 5, 7.5, 10, 15 µM.