Skip to main content
. Author manuscript; available in PMC: 2011 Aug 27.
Published in final edited form as: Immunity. 2010 Jul 30;33(2):203–215. doi: 10.1016/j.immuni.2010.07.013

Figure 1.

Figure 1

Id3−/ − CD8 thymocytes have an innate-like phenotype. (A) WT and Id3−/ − thymocytes were analyzed by flow cytometry for TCRβ . The frequency of TCRβ + cells is indicated. (B) CD4 and CD8 expression on TCRβ + cells. The frequency of CD4+ or CD8+ cells among TCRβ + cells is indicated. (C) Expression of CD122, CD44 or CD24 on WT (grey) or Id3−/ − (black) TCRβ +CD8+ thymocytes. (D) Number of TCRβ +CD8+CD122+ thymocytes in WT and Id3−/ − mice. n = 13 for each genotype. Graphs show mean +/− s.d. (E) Expression of CD122 or CD44 on WT (grey) or Id3−/ − (black) TCRβ +CD8 splenocytes. Data are representative of 10 experiments. (F) Relative expression of Eomes mRNA in Id3−/ − CD4+CD8+ or TCRβ +CD8+ thymocytes compared to WT. Graphs show mean +/− s.d. Representative of 3 experiments (G) Expression of CD44 and intracellular IFNγ in WT or Id3−/ − CD8 thymocytes 5 hours after stimulation with PMA + ionomycin. Data is representative of 3 experiments. For data on neonates see Figure S1.