Figure 2.

Id3^{−/ −} innate-like CD8 T cells were selected on MHC class Ia. (A) WT, H2-K^bH2-D^{b−/ −} or Id3^{−/ −}H2-K^bH2-D^{b−/ −} thymocytes were analyzed by flow cytometry for TCRβ . The frequency of TCRβ ⁺ cells is indicated. (B) CD4 and CD8 expression on TCRβ ⁺ cells. The frequency of CD4⁺ or CD8⁺ cells among TCRβ ⁺ cells is indicated. (C) Expression of CD122, CD44 or CD24 on H2-K^bH2-D^{b−/ −} (grey) or Id3^{−/ −} H2-K^bH2-D^{b−/ −} (black) TCRβ ⁺CD8 thymocytes. Data are representative of >5 experiments. (D) Average number (+ standard deviation) of TCRβ ⁺CD8⁺ cells in the thymus of WT, Id3^{−/ −}, H2- K^bH2-D^{b−/ −} or Id3^{−/ −}H2-K^bH2-D^{b−/ −}mice. n > 5 for each genotype, p-values are shown for the comparison of populations at the end of each line.