Figure 3.

Id3^{−/ −} innate-like CD8 T cells were selected on non-hematopoietic cells. (A–D) Flow cytometric analysis of thymocytes derived from chimeric mice 6 weeks after transplantation. (A and C) CD4 and CD8 expression on total thymocytes (upper panels), TCRβ expression on total thymocytes (middle panels) and CD4 and CD8 expression on TCRβ ⁺ thymocytes (lower panels). Expression of CD122, CD44 or CD24 on TCRβ ⁺CD8 thymocytes in (B) WT to B2m^{−/ −} chimeras (grey) or Id3^{−/ −} to B2m^{−/ −} chimeras (black) or (D) H2-K^bH2-D^{b−/ −} to WT (grey) or Id3^{−/ −}H2-K^bH2-D^{b−/ −} to WT chimeras. (E) Average number (+ standard deviation) of CD122⁺TCRβ ⁺CD8 thymocytes in chimeric mice from the indicated donor and recipient combinations. n> 3 for each combination. P-values are shown for populations at the end of each line. Three independent experiments were performed. See Figure S2 for analysis of the innate-like phenotype on Id3^{−/ −} to WT chimeras.