Figure 4.

SAP was required for the innate-like phenotype of Id3^{−/ −} CD8 thymocytes. (A) WT, Id3^{−/ −} or Sh2d1a^{−/ −} and Id3^{−/ −}Sh2d1a^{−/ −} thymocytes were analyzed by flow cytometry for TCRβ (upper) and TCRβ ⁺ cells were analyzed for CD4 and CD8 (lower). The frequency of TCRβ +cells and CD4⁺ or CD8⁺ cells among TCRβ ⁺ cells is indicated. (B) Average number of TCRβ ⁺ CD8 thymocytes in the thymus of mice with the indicated genotype. n > 6 for each genotype, p-values are shown for the comparison of populations at the end of each line. (C) Expression of CD122, CD44 or CD24 on TCRβ ⁺CD8 thymocytes from WT (grey) or mutant mice (black). (D) Average number of CD122⁺TCRβ ⁺ CD8 thymocytes in the thymus of mice with the indicated genotype. n > 5 for each genotype, p-values are shown for the comparison of populations at the end of each line. Data are representative of > 5 experiments.