Figure 6.

Unequal chimeras reveal that the innate-like phenotype was not CD8 cell autonomous. (A) Mixed bone marrow chimeras were established using an excess of Id3^{−/ −} cells (>85%, CD45.2) and a minority of WT cells (<15%, CD45.1) (left) or an excess of WT cells (>90%, CD45.1) and a minority of Id3^{−/ −} cells (<10%, CD45.2) (right). Cells were injected into irradiated CD45.2 mice. (B) TCRβ ⁺CD8 thymocytes (black line) from the majority and minority populations were analyzed for CD122 (upper), CD44 (middle) and CD24 (lower). Isotype control (grey). (C) Mixed bone marrow chimeras were established using an excess of WT cells (>70%, CD45.2) and a minority of Sh2d1a^{−/ −} cells (<30%, CD45.1) (left) or an excess of Id3^{−/ −} cells (>75%, CD45.1) and a minority of Sh2d1a^{−/ −} cells (<25%, CD45.2) (right). (D) TCRβ ⁺CD8 thymocytes (black) from the majority and minority populations were analyzed for CD122 (upper), CD44 (middle) and CD24 (lower). Isotype control (grey). Data are representative of > three experiements. See Figure S3 for experimental controls and analysis of PLZF expression.