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. Author manuscript; available in PMC: 2010 Sep 6.
Published in final edited form as: Nat Struct Mol Biol. 2006 Sep 17;13(10):921–929. doi: 10.1038/nsmb1147

Figure 2. ASF1a binding to HIRA deletion constructs.

Figure 2

(a) Binding of in vitro translated 35S-labeled HA-HIRA(421–729) and the indicated B-domain deletion mutants to 35S-labeled Myc-ASF1a.

(b) Binding of in vitro translated 35S-labeled GFP-HIRA(421–729) and the indicated C-terminal deletion mutants to 35S-labeled Myc-ASF1a.

(c) Binding of in vitro translated 35S-labeled HA-HIRA(421–729) to 35S-labeled Myc-ASF1a in the presence of 0, 1, 10 and 50µg of the wild-type HIRA peptide, RTADGRRRITPLCIA, or 50µg of the scrambled peptide, GRAARITPRDTLRCI. The height of the square and triangle indicates relative peptide concentrations.