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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Dev Neurobiol. 2010 Jan;70(1):1–15. doi: 10.1002/dneu.20750

Figure 1. RanBPM is localized to junctions at the VZ surface.

Figure 1

A, Immunoblot shows the expression of RanBPM in the E14 rat brain lysate. α-tubulin is used on the same blot as an internal control. B, Evaluation of RanBPM shRNA constructs by immunoblotting. RanBPM-shRNA1 (targeting 3’UTR region of RanBPM) is more effective than RanBPM shRNA2 (targeting coding region of RanBPM) in knocking down RanBPM expression in B104 cells. A four-base mutation construct of RanBPM-shRNA1 (RanBPM-shRNA1M4) is used as the control shRNA. α-tubulin is shown as control. C, A single confocal z section shows that RanBPM shRNA1 transfected dividing cells (left panels) decrease RanBPM on the membrane compared to the non-transfected cells (right panels). Arrowhead indicates high RanBPM expression on the cell membrane. Scale bar, 50 µm. D, RanBPM is polarized at the ventricular zone surface in E15 rat neocortex. Scale bar, 100 µm. E, Endogenous RanBPM and ZO-1 expression. E15 brain sections were immunostained with anti-RanBPM antibody and anti-ZO-1 antibody. RanBPM expression is shown as green and ZO-1 as red. F, Endogenous RanBPM (green) and β-catenin (red) expression in E15 rat brain. Scale bars, 10 µm. Insets show the dotted boxes with higher magnification. The orthogonal projected panels of single-z images confirm the co-localization of RanBPM and junctional proteins, ZO-1 and β-catenin.