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. Author manuscript; available in PMC: 2010 Sep 6.
Published in final edited form as: Cell. 2008 Oct 9;135(2):334–342. doi: 10.1016/j.cell.2008.10.002

Figure 1. Lrp4 is required for Agrin to stimulate MuSK phosphorylation.

Figure 1

(A) Agrin stimulates MuSK tyrosine phosphorylation in wild-type but not in lrp4 mutant myotubes. (A, B) Restoring Lrp4 expression in lrp4 mutant myotubes rescues Agrin-stimulated MuSK phosphorylation as well as AChR clustering. (C) Expression of LDLR fails to rescue MuSK phosphorylation. (D) Flag-Lrp4 is not tyrosine phosphorylated by Agrin stimulation, indicating that Lrp4 is not itself a substrate for MuSK. (E) A Lrp4/LDLR chimera, in which the LDLR cytoplasmic domain was exchanged for the Lrp4 cytoplasmic domain, rescues Agrin-stimulated MuSK phosphorylation and AChR clustering.