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. Author manuscript; available in PMC: 2010 Sep 6.
Published in final edited form as: Cell. 2008 Oct 9;135(2):334–342. doi: 10.1016/j.cell.2008.10.002

Figure 4. Lrp4 self-associates and forms a complex with MuSK.

Figure 4

(A) BaF3 cells, as well as BaF3 cells expressing myc-MuSK-GFP or CCR7-GFP, grow in suspension as single cells. Flag-Lrp4-mCherry-expressing cells, however, form aggregates, indicating that Lrp4 binds Lrp4. Although myc-MuSK-GFP-expressing cells grow as solitary cells when cultured alone, they co-aggregate with Flag-Lrp4-mCherry expressing cells, indicating that MuSK binds Lrp4. (B) Binding between Lrp4 and MuSK is specific, since myc-MuSK-GFP-expressing cells fail to aggregate with themselves, and BaF3 cells expressing CCR7-GFP fail to co-aggregate with Flag-Lrp4-mCherry-expressing cells. (C) Lrp4 and MuSK interact in cis, as they co-immunoprecipitate when expressed together in the same cell.