. 2010 Jun 18;67(19):3219–3240. doi: 10.1007/s00018-010-0419-2

Table 2.

Modelling distinct modes of tumour migration

Environment	Models	Microscopy	Obtained information	References
In vitro/2D	Matrix coating	Wide-field, confocal	Insights into the organisation of molecular machineries underlying cell adhesion and migration	[24, 53, 120, 129, 159–163]
In vitro/2D	Patterned	Wide-field, confocal		[24, 53, 120, 129, 159–163]
In vitro/3D	Matrigel	Wide-field, confocal, confocal reflection microscopy, SHG	Distinguish aspects of cell movement/invasion (collective/individual; mesenchymal/amoeboid). Visualise interactions cell. ECM (in particular, collagen fibers I)	[25, 109, 110, 112–114, 116–118, 121, 132, 159, 164]
In vitro/3D	On/in collagen gel	Wide-field, confocal, confocal reflection microscopy, SHG		[25, 109, 110, 112–114, 116–118, 121, 132, 159, 164]
In vivo	Zebrafish	Confocal, confocal reflection, Multiphoton (SHG and FLIM)	Aspects of cell movement in the primary environment. Visualise interaction between tumour cells and tumour environment (ECM, host cells and blood vessels). Visualise intravasation event when blood vessels are counterstained	[1, 49, 120, 123, 125–135, 165–171]
	Mouse
	Rat