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. 2010 Jul 23;11(9):691–697. doi: 10.1038/embor.2010.99

Figure 1.

Figure 1

Metastasis-associated protein 1 is acetylated on Lys 626. (A) ZR-75 cells were treated with or without TSA (8 h) and cell lysates were probed with the MTA1-AcK626 or MTA1 antibodies. The asterisk indicates an acetylated MTA1 band. (B) ZR-75 cells were treated with and without TSA, fixed in paraformaldehyde and MTA1-AcK626 antiserum was used to perform immunofluorescence. An increased signal with TSA treatment is apparent for MTA1-AcK626 antibody. The signal with the antiserum could be effectively competed out using the Lys 626 peptide. (C,D) Cancer cells were treated with control, p300 or PCAF siRNA for 48 h and cell lysates were probed with the indicated antibodies. (E) Lysates from exponentially growth indicated cell lines were probed with antibodies against MTA1-AcK626, MTA1, p300, PCAF or actin. MTA1, metastasis-associated protein 1; PCAF, p300/cAMP response element-binding protein-associated factor; siRNA, small interfering RNA; TSA, Trichostatin A.