Figure 3.

DNA damage-induced foci formation of GFP-Rad9. (a) GFP-Rad9 expressing cells were left untreated or exposed to UV, IR, or aphidicolin. GFP-Rad9 was detected by direct fluorescence and RPA and γH2AX by immunofluorescence. (b) GFP-Rad9 expressing cells were treated with 40 J/m² local UV-irradiation using an isopore filter. One hour later the cells were fixed and stained. GFP-Rad9 was detected by direct fluorescence and TFIIH by immunofluorescence.