Fig. 10.

Effect of thiabendazole on growth and nuclear number in U. maydis wild type and lis1-depleted cells. A. Wild type and lis1-depleted cells were grown on UMC arabinose (1–3) or glucose (4–6) agar with 0 (1 and 4), 2 μg/mL (2 and 5), or 5 μg/mL (3 and 6) TBZ. Strains in 1–3: a = FB1 and b = FB1 Pcrg1∷lis1; 4–6, a = FB1 Pcrg1∷lis1 and b = FB1. Similar observations were made with independent Pcrg1∷lis1 isolates in the FB1 and SG200 strain backgrounds. B. Average number of nuclei in wild type (black bars) and lis1-depleted cells (white bars) grown in MM glucose broth containing DMSO, or 2 or 5 μg/mL TBZ for 39–45 h and stained with DAPI. (See Supplemental table III for cell and nuclear counts.) C. Percent of wild type cells with one or more nuclei (See Supplemental table III). DMSO (black bar); 2 μg/mL TBZ (white bar); and 5 μg/mL TBZ (gray bar). Cells were grown as described in B. D. Percent lis1-depleted cells with one or more nuclei (See Supplemental table III). Bars equal bars in C. Cells were grown as described in the legend to B.