Fig. 5.

Time-lapse analysis of cell morphology and budding pattern in U. maydis wild type cells. Sequence of photomicrographs showing cell morphology of wild type cells through multiple cycles of budding of a mother cell and its daughters. The mother cell (M) forms four buds (1, 2, 3, 4) from the same cell pole and no buds from the opposite pole during this sequence (t = 0 to t = 6.75 h). Daughter cell 1 buds twice (1-1 at t = 3 h and 1-2 at t = 5:15 h) from the distal pole (opposite the birth site), and daughter 1-1 buds distally too (1-1-1 at t = 6 h). Daughter cell 2 also buds distally (2-1 at t = 5 h). Daughter cell 3 buds proximally (3-1 at t = 6 h). From bud initiation to bud separation is approximately 2 h. Arrows in the penultimate sequence point to emerging buds that are partially masked by other buds. Cell morphology remained unaltered in this and longer sequences. Time in hours is indicated on the bottom right hand of each panel. (See Supplemental video 1.) Bars = 5 μm.