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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Gastroenterology. 2010 Jun 4;139(3):942–952. doi: 10.1053/j.gastro.2010.05.083

Figure 3. Spontaneously transformed ICC stem cells transplanted into NCr-nu/nu mice give rise to malignant GIST-like tumors.

Figure 3

(A) Tumor formed in a 2xSCS70-transplanted mouse 43 days after grafting. Scales are in centimeters. (B) Hematoxylin-eosin. Magnification is 400x. (C) Kit immunoreactivity (red; blue: DAPI) in tumors detected with a rabbit polyclonal antibody. The third panel from left is an enlargement of the area outlined in the preceding panel. Arrowhead indicates a bipolar, ICC-like cell. Arrows show dividing ICC-like cells. (D) Secondary-antibody-only control for C and F. (E) Kit immunoreactivity detected with rat monoclonal antibody 2B8. Right panel shows donor skin tissue lacking Kit immunostaining (asterisk) in the vicinity of the tumor as control. (F) Ano1 immunoreactivity. Middle panel is an enlargement of the area outlined in the left panel. Arrowheads indicate bipolar, ICC-like cells. Right panel shows Ano1+ tumor cells infiltrating host skeletal muscle (asterisks). White and yellow scale bars are 50 μm and 12.5 μm, respectively. Note moderate, generalized Kit and Ano1 immunostaining and high Kit and Ano1 expression in ICC-like cells and in some round cells.