Design and synthesis of Hsp90 Inhibitors: exploring the SAR of Sansalvamide A derivatives

. Author manuscript; available in PMC: 2011 Sep 15.

Published in final edited form as: Bioorg Med Chem. 2010 Jul 22;18(18):6822–6856. doi: 10.1016/j.bmc.2010.07.042

*Instrument:* Waters Flex Inject Waters 2487 Dual λ Absorbance Detector
*Column:* Symmetry C₁₈ 3.5μm 4.6×75mm Column
*Mobile Phase A:* 0.1% (v/v) Trifluoroacetic acid, 100% (v/v) water
*Mobile Phase B:* 0.1% (v/v) Trifluoroacetic acid, 100% (v/v) acetonitrile
λ₁: 215nm
λ₂: 222nm
*Gradient:*	Time (min)
	Profile %A	Profile %B
	0	70
	30
	4.00	0
	100
	13.00	0
	100
	15.00	70
	30
	16.00	70
	30
*Flow rate:* 0.50 ml/min
*Injection:* 20μL
*Solvent:* 100% Methanol