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. Author manuscript; available in PMC: 2011 Oct 15.
Published in final edited form as: Biochem Pharmacol. 2010 Jul 1;80(8):1274–1283. doi: 10.1016/j.bcp.2010.06.043

Fig. 1. Induction of rat PXR in primary hepatocytes by dexamethasone.

Fig. 1

Primary hepatocytes from Sprague-Dawley male rats (8-week old, n = 4) were isolated by a modified two-step collagenase digestion method. Hepatocytes were initially seeded and cultured in Williams’ E medium for 48 h with replacing the medium at 24 h. Thereafter, hepatocytes were cultured in dexamethasone-free medium for 16 h and then treated with dexamethasone at various concentrations (0–10 μM). Then hepatocytes were harvested for preparing cell lysates and total RNA. Total RNA was analyzed for the level of PXR mRNA by RT-qPCR and cell lysates (10 μg) were analyzed for the levels of PXR or CYP3A23 proteins by Western blotting. The RT-qPCR signals were normalized based on the abundance of Pol II mRNA. (A) Induction of PXR as a function of concentrations, and (B) Time-course study on the induction of PXR. The Ct value (threshold cycles) for DMSO controls in all time-points is around 25.65. *Statistically significant difference (p < 0.05).