Table 1.

Sequences of primers and probes used in real time RT-PCR

Receptor	Primer	Sequence (5′→3′)
Gag	Sense	GTATGATCGTACTCATCCTCCTGAT
	Anti-sense	TCTACATTGCATTCTGGCTGGT
	Probe	AGACCACTGCCCTACTTCACTGCCG
β-actin	Sense	GACTACCTCATGAAGATCCTCACG
	Anti-sense	CCTTGATGTCACGCACAATTTCC
	Probe	ACAGTTTCACCACCACCGCCGAGC
FOXP3	Sense	GCCTGCCACCTGGAATCAAC
	Anti-sense	GTGTGCTGGGGCTTGGGA
	Probe	CAGTGCTGGCTCCCTGGACACCCA
CD25	Sense	CCACGTGACAGAACTGTGTG
	Anti-sense	GTGCCCGTCTTGTATGTGAG
	Probe	CCGCCAGATATCCAACACGCC
CTLA4	Sense	TCTCCAAAGGGATGCATGT
	Anti-sense	CCCATATTCACACACGAAGC
	Probe	CTGGCCAGCACCACTGCAGG

Target probe labels: 5′ FAM (6-carboxyfluorescein), 3′ TAMRA (6-carboxytetramethylrhodamine).

β-actin labels: 5′ HEX (hexachloro-6-carboxyfluorescein), 3′ TAMRA.