Table 3.

Correlation of FOXP3 and CTLA4 co-expression in individual tissues

Early Pregnancy
Tissue No. (FIV neg)	FOXP3	CTLA4	Tissue No. (FIV pos)	FOXP3	CTLA4
6108 A	↓	↓	6062 A	↓	↓
6108 B	↓	↑	6062 B	↑	↓
7284 A	↓	↑	5111 A	↓	↓
7284 B	↓	↓	5111 B	↓	↓
8059 A	↓	↓	5111 C (NV)	↓	↓
8059 B	↑	↓	0866 A	↓	↓
2779 A	↑	↑	0866 B	↓	↓
2779 B	↑	↑	1126 A	↓	↓
2779 E (NV)	↓	↓	1126 B	↓	↑
3550 A	↑	↓	0326 A	↓	↓
3550 C	↑	↓	8035 A	↑	↓
9276 A	↓	↑	8035 B (NV)	↓	↑
9276 B	↓	↓	8035 C (NV)	↓	↓
9276 F (NV)	↑	↑	8035 D (NV)	↓	↓
8291 A	↑	↑	1893 A	↓	↓
8291 B	↓	↑	1893 B	↓	↑
0373 A	↓	↓	1893 C (NV)	↓	↑
0373 B	↓	↑
Both markers decreased: 6/18 (33%)			Both markers decreased: 11/17 (65%)
Both markers increased: 4/18 (22%)			Both markers increased: 0/17 (0%)
No correlation: 8/18 (44%)			No correlation: 6/17 (35%)
Chi2= 1.333; P= 0.513			Chi2= 10.727; P= 0.005
Late Pregnancy
Tissue No. (FIV neg)	FOXP3	CTLA4	Tissue No. (FIV pos)	FOXP3	CTLA4
9522 A	↓	↓	9674 A	↓	↑
9522 B	↑	↑	9730 B	↑	↑
9581 A	↑	↓	9745 B	↑	↑
9581 B	↓	↓	9745 A (NV)	↑	↑
9746 A	↑	↓	9745 C (NV)	↑	↑
9746 B	↑	↓	9745 E (NV)	↓	↑
9801 A	↑	↑	9745 F (NV)	↓	↑
9801 B	↓	↑	9806 C	↓	↑
13634 A	↓	↑	9809 A	↑	↑
13668 A	↓	↑	9810 A	↓	↑
13668 B	↑	↑	9813 A	↑	↓
13671 A	↓	↑	13226 A (NV)	↓	↑
13671 B	↓	↑	13226 B (NV)	↑	↑
			13226 C (NV)	↓	↓
			13226 D (NV)	↓	↑
			13226 E (NV)	↑	↑
Both markers decreased: 2/13 (15%)			Both markers decreased: 1/16 (6%)
Both markers increased: 3/13 (22%)			Both markers increased: 7/16 (44%)
No correlation: 8/13 (62%)			No correlation: 8/16 (50%)
Chi2=4.749; P =0.093			Chi2=5.366; P= 0.068

Tissue numbers = queen number followed by alphabetical designation of fetus (NV) = non-viable fetus

Increased (↑) or decreased (↓) expression is relative to mean Ct values for tissue expression of each marker obtained from control (FIV neg) cats.