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. 2001 Feb 13;98(4):1817–1822. doi: 10.1073/pnas.98.4.1817

Figure 6.

Figure 6

Caspase 1 degrades p40. (A) Schematic representation of caspase 1 cleavage site in p40. (B) p53-expressing lysates contain caspase 1, which cleaves p40 in vitro. Total lysates from SaOs2 cells infected with Ad-p53 were incubated with [35S]p40 or 35S-labeled mutated p40 (YVEA185) in the absence or presence of 5 μM each caspase substrate–inhibitors (for caspase 1, Ac-YVAD-CHO; caspase 3, Ac-DEVD-CHO; caspase 6, Ac-VEID-CHO; caspase 8, Ac-IETD-CHO). (C) Purified caspase 1 cleaves p40 in vitro. For protease cleavage, [35S]p40 or 35S-labeled mutated p40 (YVEA) polypeptides were incubated with purified caspases for 2 h at 37°C and analyzed by SDS/PAGE followed by autoradiography. p40 (residues 1–356, 44 kDa) is cleaved in half, generating two fragments (residues 1–184 and 185–356) with an apparent molecular mass of 20 kDa.