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. 2001 Feb 13;98(4):1823–1828. doi: 10.1073/pnas.98.4.1823

Figure 2.

Figure 2

DIP-thrombin-induced aggregation is inhibited by anti-GPIb antibody. WP from GP V null mice were preincubated with either anti-GPIb–IX Ab 3584 (2 μM, line 2) or control rabbit IgG (2 μM, line 1) for 10 min at room temperature. (A) Aggregation was initiated by the addition of DIP-thrombin (100 nM). (B) Aggregation was initiated by the addition of thrombin (10 nM). (C) Glycocalicin-purified Ab 3584 IgG (1.89 μM) was incubated with GP V null platelets for 10 min before the initiation of aggregation with 500 nM DIP-thrombin (line 2). Control (line 1) shows the aggregation response with 500 nM DIP-thrombin after incubation with control rabbit IgG. (D) GP V −/− WP were preincubated with either control IgG (2 μM, line 1) or Ab 3584 (2 μM, line 2) for 10 min. Aggregation was initiated by the addition of 1 μM S205A-thrombin.