Figure 3.

Effect of various inhibitors on aggregation. Pooled WP were treated with the indicated concentrations of unfractionated heparin (0.3 unit/ml, A–D), or PGI₂ (4.45 μM, E–H), and aggregation responses were measured compared with that obtained in the absence of heparin or PGI₂. GP V null WP aggregated with 500 nM DIP-thrombin (A, E) or 10 nM thrombin (B, F) and thrombin-pretreated wt platelets aggregated with 500 nM DIP-thrombin (C, G) or wt platelets aggregated with 10 nM thrombin (D, H). Line 1 in each tracing represents the untreated control platelets and line 2 represents the platelets treated with inhibitor. The tracings are typical of results from experiments performed at least three times with pooled platelets from four mice each.