(a) The time-course of the effects of ovariectomy in
normal rats on the pituitary LH-RH-R mRNA expression, serum LH, and
pituitary LH concentration. (b) RT-PCR products of the
pituitary LH-RH-R mRNA and β-actin mRNA, separated by agarose gel
electrophoresis and stained with ethidium bromide. PCR products were
the expected size of 441 bp (LH-RH-R) and 542 bp (β-actin). Lane M,
100-bp DNA molecular weight marker; lanes 1, 2, and 3, normal rats;
lanes 4, 5, and 6, OVX rats after 11 days; lanes 7, 8, and 9, OVX rats
after 21 days. **, P < 0.01 vs.
initial values.