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. 2001 Jan 30;98(4):1829–1834. doi: 10.1073/pnas.031582398

Figure 5.

Figure 5

In vitro effects of Cetrorelix on LH-RH-R expression and LH response in normal and OVX rat pituitary cells in the superfusion system. (a) LH-RH-R mRNA and β-actin mRNA expression of normal pituitary cells perfused continuously with 100 nM Cetrorelix for 10 h. RT-PCR products are shown after separation by agarose gel electrophoresis and staining with ethidium bromide. Lane M, 100-bp DNA molecular weight marker; lanes 1 and 2, Cetrorelix-treated; lanes 3 and 4, untreated control cells. (b) LH responses of normal rat pituitary cells to 1 nM LH-RH pulses (L) for 3 min at 30-min intervals before, during, and after a continuous superfusion with 20 nM Cetrorelix for 2 h (horizontal bar). (c) LH responses of pituitary cells from OVX rats to 1 nM LH-RH pulses (L) for 3 min at 30-min intervals before, during, and after a continuous superfusion with 20 nM Cetrorelix for 2 h (horizontal bar). (d) LH responses of control cells from OVX rats to 1 nM LH-RH pulses for 3 min at 30-min intervals (L).