Table 3.

Advantages and disadvantages of density gradient centrifugation.

Advantages	Disadvantages
- usually clean fraction of highly motile spermatozoa	- production of good interphases between the different media is a bit more time-consuming
- spermatozoa from ejaculates with a very low sperm density can be separated	- a bit more expensive
- good yield	- potential risk of endotoxins
- leukocytes are eliminated to a large extent	- Percoll® may no longer be used IVF/ICSI
- reactive oxygen species are significantly reduced