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. 2001 Feb 6;98(4):1847–1852. doi: 10.1073/pnas.041599798

Figure 5.

Figure 5

sGC and PKG are indispensable for the induced expression of the γ-globin gene by Hb F-inducing agents. (A) Induced expression of the γ-globin gene by hemin or butyrate is abolished by an sGC inhibitor. Cells were treated with 1 μM LY83583 (sGC inhibitor) for 12 h and then incubated with hemin or arginine butyrate for 3 days. (Left) Northern blot. Lanes M, molecular weight marker; lane 1, control; lane 2, 1 μM LY83583; lane 3, 20 μM hemin; lane 4, 1 μM LY83583 + 20 μM hemin; lane 5, control; lane 6, 1 mM arginine butyrate; lane 7, 1 μM LY83583 + 1 mM arginine butyrate. (Right) Comparison of mRNA levels. *, P < 0.001. (B) A selective PKG inhibitor inhibits the induction of γ-globin gene expression by hemin or butyrate. Cells were treated with 8 μM KT5823 (PKG inhibitor) for 12 h and then incubated with hemin or butyrate for 3 days. (Left) Northern blots. Lanes M, moecule weight markers; lane 1, control; lane 2, 8 μM KT5823; lane 3, 20 μM hemin; lane 4, 8 μM KT5823 + 20 μM hemin; lane 5, 1 mM arginine butyrate; lane 6, 8 μM KT5823 + 1 mM arginine butyrate. *, P < 0.001; **, P < 0.05.