TABLE 3 .
Size distribution of H3N2 leader RNAsa
Length (nt) | No. of leRNAs of the indicated length derived from the following influenza vRNA segment: |
|||||||
---|---|---|---|---|---|---|---|---|
PB2 | PB1 | PA | HA | NP | NA | M | NS | |
18 | 33,567 | 8,512 | 15,097 | 6,858b | 4,498 | 14,251 | 7,049 | 6,858b |
19 | 115,343 | 10,093 | 15,888 | 15,330b | 13,470 | 6,462 | 28,056 | 15,330b |
20 | 36,539 | 18,962 | 34,487 | 3,742b | 24,710 | 21,361 | 9,064 | 3,742b |
21 | 70,309 | 28,898 | 70,226 | 7,308b | 42,140 | 9,728 | 26,498 | 7,308b |
22 | 82,197 | 12,056 | 105,011 | 7,526 | 46,306 | 8,184 | 16,255 | 5,247 |
23 | 22,272 | 20,949 | 63,814 | 2,878 | 48,082 | 15,643 | 11,174 | 6,664 |
24 | 42,376 | 16,307 | 26,101 | 5,006 | 42,786 | 14,607 | 1,500 | 2,296 |
25 | 32,327 | 34,429 | 24,996 | 14,640 | 42,608 | 15,033 | 2,002 | 7,152 |
26 | 75,262 | 32,694 | 8,399 | 5,673 | 12,353 | 3,100 | 3,346 | 5,094 |
27 | 51,910 | 9,173 | 9,574 | 3,125 | 5,053 | 4,798 | 326 | 3,745 |
Total | 562,102 | 192,073 | 373,593 | 72,085 | 282,006 | 113,167 | 105,270 | 63,435 |
This table presents the size distribution, in nucleotides, of the leRNAs derived from each of the indicated influenza vRNA segments, with the most prevalent size in boldface type.
Because the HA and NS vRNAs are identical in sequence through nucleotide 21, we could not assign reads derived from these sequences unequivocally. We have therefore arbitrarily assigned 50% of the reads to each of these two segments.